Manganese Superoxide Dismutase Gene Ala16Val Polymorphism in Pulmonary Tuberculosis Patients with Diabetes at Balai Pengobatan Lung Disease, Medan City

Abstract

Manganese Superoxide dismutase (Mn-SOD) gene Ala16Val polymorphism has been shown to be associated with several infectious disease and metabolic disorder. This study to analyze Mn-SOD gene Ala16Val polymorphism in patients with pulmonary tuberculosis (PTB) and also suffer from diabetes mellitus (DM). The study was conducted at Balai Pengobatan Lung Disease, Medan and Integrated Laboratory of Faculty of Medicine, University of Sumatera Utara (USU), Medan. Mn-SOD gene Ala16Val polymorphism was studied in 40 outpatient at Balai Pengobatan Lung Diseases, Medan City. The blood glucose was measured with a spectrophotometer, using commercial glucose kits. Polymerase chain reaction-restriction fragment lengths polymorphisms (PCR-RFLP) technique was done with the BsaW1 restriction enzyme. SPSS version 22 was used for statistical data. Ala and Val were analyzed to see the frequency of genotype of Mn-SOD gene 16Ala/Val polymorphism by direct counting. Hardy-Weinberg Equilibrium was analyzed by a Chi-square test. The results show that the percentage of Val/Val genotype (57.5%) of Mn-SOD gene was higher compared Ala/Val and Ala/Ala genotypes (37.5%, 5 %) in PTB patients with diabetes. This preliminary study results indicated that Val/Val genotype was common genotypes in the Mn-SOD gene in PTB patients with diabetes at Balai Pengobatan Lung, Medan city.