Synthesis and Evaluation Antibacterial Activity of Phosphate Buffer Solution (pH 7.4) - Soluble Acylated Chitosan Derivative
Pasaribu, Subur P.
Sinaga, Kasmirul Ramlan
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The N-maleoyl chitosan (MCS) is a chemically modified derivative of the biopolymer chitosan that was synthesized through acylation reaction between chitosan and maleic anhydride acid. The resulting MCS was characterized by FT-IR spectroscopy and its antibacterial activity was evaluated against Staphylococcus aureus (Gram- positive) and Escherichia coli (Gram-negative) bacteria by Agar diffusion method on the Muller Hinton Agar. MCS was soluble in a phosphate buffer solution (PBS, pH 7.4) with a concentration of 2 % (w/v), and did not show any antibacterial activity against both test bacteria. However, with the formation of chitosan derivative soluble in the physiological medium of PBS, pH 7.4 will expand its utilization in the formation of hydrogels for biomedical applications such as drug delivery.