Antibacterial Effectiveness of Noni Extract (Morinda Citrifolia L.) on the Growth of Fusobacterium Nucleatum (ATCC® 25586™) as an Alternative Material for Root Canal Irrigation- An In-Vitro Study
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BACKGROUND Irrigation solution is needed to lower the number of microorganisms in the root canal. Irrigation solution commonly used have flaws. It required the development of alternative irrigation materials from natural ingredients, one of which is the fruit of noni because it has antibacterial properties and is a biocompatible bonding agent. This research aims to know the antibacterial effect of the ethanol extract of the fruit of nonito on Fusobacterium nucleatum by determining the value of the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). METHODS The research starts with drying noni fruit (3 kg) and obtained 400-gram of simplisia extracted with 5 L of ethanol 70% solvent, then evaporated to 50 grams. Antibacterial test was done using the method of dilution and diffusion. Dilution method- determination of turbidity was done by observing the MIC tubes visually and compared with control McFarland after being incubated for 24 hours at a temperature of 370C, then amount of bacteria was calculated to get a value of MBC. Diffusion methods- using paper discs that absorb extract. Drag zone formed around the disk is measured with a ruler. Data obtained from this study is analysed with the test of Kruskal-Wallis and Mann-Whitney. RESULTS In dilution method, the tube starts to look crystal clear at a concentration of 12.5% and the absence of bacteria at a concentration of 25% to 100%. In diffusion method, the smallest zone was seen at concentrations of 12.5%. CONCLUSIONS Ethanol extract of noni fruit has antibacterial effects against Fusobacterium nucleatum MIC 12.5% to the value and the value of the MBC 25%.