Antibacterial Activity of Betle Leaf (Piper betle l.) Extract on Inhibiting Staphylococcus aureus in Conjunctivitis Patient
Abstract
The aim of this study is to determine the antibacterial activity of Piper betle L. leaf extract on inhibiting
Staphylococcus aureus in conjunctivitis patient. This study follows a post-test only group experimental design.
Antibacterial activities of five Piper betle L. extract concentrations (0.5%, 1%, 1.5%, 2%, 2.5%, and 3%) against
Staphylococcus aureus were evaluated by agar well diffusion method. The negative control group (P-) was treated
with standard 10% DMSO solution, the positive control group (P+) with ceftriaxone. The diameters of clear zone surrounding
the well were analyzed with Kruskal-Wallis test and showed that in between 1% and 1.5%; 1.5% and 2%;
2% and 2.5%; 2.5% and 3% concentrations do not show a significant difference but in between 0.5% and 1%; 0.5%
and 1.5%; 0.5% and 2%; 0.5% and 2.5%; 0.5% and 3%; 1% and 2%; 1% and 2.5%; 1% and 3%; 1.5% and 2.5%;
1.5% and 3%; 2% and 3% concentrations, Piper betle L. leaf extract shows a significant difference on inhibiting the
growth of Staphylococcus aureus. In conclusion the results obtained show that that Piper betle L. leaf extract has a
significant potential use as an antibacterial agent.