Pap Smear cytology and Identification of Human Papillomavirus (HPV) Type 16 and 18 in Multiparity Women at Aviati Clinic Padang Bulan Medan
Anggraini, Dwi Rita
Fitrie, A A
Ginting, R N A
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Cervical cancer is the second most frequent cancer in woman in developing countries and one of the most crucial health problems in the world. Human Papillomavirus (HPV) is an agent for sexually transmitted disease which is an act of cervical cancer, especially high-risk of HPV type 16 and 18. In this study, we investigated the Pap smear cytology features and identification of HPV types 16 and18 in multiparity women at Aviati Clinic Padang Bulan, Medan. Samples are cervical swabs of 50 multiparity women who met the inclusion criteria (childbirth . three times) was included in the study. Pap smear examination was conducted using Papanicolaou staining and identification of HPV types 16 and 18 using the Polymerase Chain Reactive (PCR) methods. Pap smearcytology showed 80% Negative for intraepithelial lesion or malignancy (NILM) with inflammation and 20% NILM. The result of PCR amplification showed that there weren't specific band DNA was found at band 414bp and 216bp. That means there weren't cervical swabs sample had DNA of HPV type 16 and 18.