Optimization of Enzymatic Process in Biodiesel Production for Green Process Development
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Biodiesel production by using conventional base trans esterification or other chemical processes is generally selected as an effective way. But besides, an enzymatic process in biodiesel production is more biodegradable and has many advantages compared to the conventional process. The most important advantage in the enzymatic process is a green technology and safe for the environment. In this study, N ovozyme 435 was used as immobilized lipase with degwnmed crude pahn oil (as known as Degwnmed Palm Oil (DPO as raw material. The aim of this study was to determine the optimwn condition of the enzymatic process in obtaining the maximwn yield percentage by using mathematical statistical analysis and designed as Response Surface Methodology (RSM) method. The reaction was performed at various methanol molar ratio to oil, temperature, reaction time and stirring speed. The optimwn reaction condition obtained was 8 h of reaction time at 4S0C of temperature, a molar ratio ofDPO: methanol was 9: 1, the amollllt of enzyme was 20% of the DPO and ISO rpm of stirring speed.