The Role of FokI Polymorphism of Vitamin D Receptor Gene and Vitamin D level in Multidrug-Resistant Tuberculosis Occurrence in Medan city, Indonesia
Sinaga, Bintang Yinke Magdalena
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Introduction: Multidrug-resistant tuberculosis (MDR-TB) has become a new problem in the world. Many factors have been associated with MDR-TB occurrence. Some studies have demonstrated a role of vitamin D level and vitamin D receptor (VDR) gene polymorphism in MDR-TB. The aim of this study is to explore the role of FokI polymorphisms of the VDR gene and vitamin D level in MDR-TB occurrence in Medan city, Indonesia. Material and methods: This is a case-control study. Cases were 43 MDR-TB patients from the MDR-TB polyclinic Adam Malik Hospital in Medan, Indonesia. The control group comprised 56 new pulmonary TB cases with positive AFB sputum smear. Patients who were HIV positive, known to have diabetes mellitus or other severe disease, taking immunosuppressive drugs and vitamin D were excluded for both groups. Genetic polymorphisms of the VDR gene were analyzed using PCR-RFLP. Vitamin D level was analyzed using the ELISA procedure. Results: The frequencies of FokI genotypes were FF 39.5%, Ff 53.5%, ff 7.0% for MDR-TB patients and FF 39.3%, Ff 46.4.% and ff 14.3% for controls. There was no significant association between FokI genotype of the VDR gene and MDR-TB. Vitamin D level in MDR-TB was 53.5% sufficient, 39.5% insufficient and 7.0% deficient. Vitamin D level was 41.1% sufficient, 46.4% insufficient and 12.5% deficient in TB non-MDR. There was no significant association between vitamin D level and MDR-TB. Conclusions: No role of FokI polymorphism of the VDR gene and vitamin D level in MDR-TB occurrence was found.