Diagnostic Value of Nested-Pcr for Identification of Uaiassezia Species in Dandruff
Jusuf, Nelva Karmila
Nasntion, T A
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Abstract. Dandruff or pityriam simplex is a condition of abnormal occurrence of formation of ydhwish white scales from the scalp Many factors play a role in the pathogenesis of dandruff, i c colcaiatkm of XLilazscru species. Eamanatian of Malaxsma species previously done by culture as the gold standard However, there arc vanous difficulties in doing the culture Identification method with ancsted-poiymcrase chain reaction |ncsled-PCR> u expected to provide quickly and easily detected. This study a medio determine the diagnostic value of nested-PCR in the identification of Slulasscxa species at dandruff From 21 subjects, scales from the scalp were taken and sent to the laboratory for nested-PCR identification Statistical analysis of diagnostic test earned out to determine sensitivity, ipccificitv. positive predictive value, and negative predictive value. The result mowed nested-PCR detected 10 sample (47.6*.| positive for Mataaezui species consist of If nmpodtalu (23.8". t; If tiooftae (9.5*.C .If furfur <4.8«.fc if gfobosa and .W furfur <4 §*.(: and If restntla and .1/ nmpodulu <4.R*.». Detection of SLhwau species by nested-PC R has 100*« in sennuvity whereas the specificity wus 55". Nested-PCR test has high sensitivity Therefore nested-PCR may be considered for a faster and simpler alternative examination in identification for MJIOXSCM species in dandruff.