Deteksi dan Serotiping Virus Dengue dan Serum Penderita Demam Dengue di Medan Menggunakan Reverse Transkriptase PCR
Kusumawati, R. Lia
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Introduction: Detection of dengue virus infections is important for clinical management, surveillance and clinical trial assessments. Reverse transcriptase-PCR (RT-PCR) amplification of dengue viral RNA can offer a rapid, sensitive, and specific approach to the typing of dengue viruses. Early detection of dengue virus in patient serum allows the possibility of mounting a rapid response aimed at vector control in the affected areas. This study aimed to describe the frequency of serotypes 1, 2, 3 and 4 of dengue virus in serum samples obtained from patients clinically suspected of having dengue fever or dengue hemorrhagic fever in Medan. Method: Reverse transcriptase-PCR (RT-PCR) used to determine the dengue virus serotype serum collected from human patients with dengue in acute phase. Results: Of 100 serum samples from acute phase of DF/DHF patients as WHO 1997 clinical criteria, 74 (74%) were infected by Dengue serotype 2 (DEN-2), 2 (2%) were infected by DEN-1, and 24 (24 %) serum samples were negative by RT-PCRRT PCR. In this study, the mostly patients infected by dengue viral age were between 15-44 years old (64.48 %). Conclusions: This assay is useful for typing the virus and providing important information to provide epidemiologic information for surveillance of circulating dengue viruses.