Antidiabetic Activity Test Of Ethyl acetate Extract Of Poguntanoh Leaves (Curanga fel-terrae Merr)
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Background: Poguntanoh (Curanga fel-terrae Merr) is a species of Scrophulariaceae, it has been used to treat diabetes mellitus traditionally and give satisfactory results. Objective: The Research was intended to know in vivo antidiabetic activity of ethyl acetate extract of plant leaves in white male mice. Methods: The research include characterization, phytochemical screening of simplex, ethyl acetate extact of poguntanoh (Curanga fel-terrae Merr) leaves, testing in vivo antidiabetic activity performed in white male mice with alloxan induction method. Test of the antidiabetic effect of ethyl acetate extract of Curanga fel-terrae Merr. leaves was conducted by using alloxan induced diabetic mice by single dose of ethyl acetate extract. Diabetic Mice were divided into 3 groups and each group consisting of 6 mice. Group I were given suspension of 1,0 % CMC-Na, dose 1 % bw, Group II were given suspension of Metformin® with dose 50 mg/kg bw and Group III were given suspension of ethyl acetate extract of. Curanga fel-terrae Merr, Leaves with dose 200 mg/kg bw. Suspension of test material (extract) was administered for 5 consecutive days orally and the BGL of mice were measured on the third, fifth, seventh, and tenth days after administration of the test material10,15,17. Results: The results of the characterization of simplex and ethyl acetate extract of poguntanoh leaves (Curanga fel-terrae Merrr) obtained water content 5.97%, 4,91%, respectively. Content of water soluble 25.66%, 0,50%, respectively. Content of ethanol soluble 19.32%, 0.08% respectively. Total ash content 4.01%, 13,94%. respectively. Content of acid insoluble ash 0.78%, 61,39%, respectively. Phytochemical screening of simplex showed the presence of flavonoid, glycosides, saponins, tannins and steroids/triterpenoids and ethyl acetate extract showed the presence of flavonoid, glycosides, saponins, tannins. Blood glucose reduction of test animals after treated with ethyl acetate extract up to tenth days was 53.96% All of the data were analyzed statistically by analysis of variance (ANOVA) method, using SPSS (Statistical Product and Service Solutions) 17.0 software Conclusion: Ethyl acetate extract of Curanga fel-terrae Merr, Leaves is effective as antidiabetic agent, ethyl acetate extract have ability to reduce BGL 53,96% up to tenth days.