Isolation of β-sitosterol from n-Hexane Extract of Picria fel-terrae Lour. Leave and Study of Its Antidiabetic Effect in Alloxan Induced Diabetic Mice
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Picria fel-terrae Lour., is an effective antidiabetic agent empirically. β-sitosterol is one of the steroid class of chemical constituents contained in the n-hexane extract of leave Picria fel-terrae Lour. The purpose of this study are to extract Picria fel-terrae Lour. leaves with n-hexane and study its antidiabetic effect and isolation of β-sitosterol from n-hexane extract. Structure elucidation of β-sitosterol was conducted by using spectroscopic methods (1H and 13C NMR, IR, and MS). N-hexane extract of Picria fel-terrae Lour. leaves shows good results as an antidiabetic agent, indicated by significant BGL depletion of alloxan induced diabetic mice for ten days, i.e. 44.47%, with metformin 50 mg/kg as a comparison. Based on the results of the analysis, the isolate A1 is steroid group, 24-ethyl-5α-cholest-5-en-3β-ol (β- sitosterol).