The Effect Of Curcuminoid To Noise Exposure Viewed From The Expression Of Heat Shock Protein-70 (HSP-70) In Cochlear Fibroblasts Of Rattus Norvegicus
Haryuna, Tengku Siti Hajar
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Numerous epidemiological and experimental studies have been carried out so far, from which empirical evidences were obtained and various theories were suggested to elucidate a series of Noise-Illduced Hearing Loss (NIHL) process at the molecular level. One of them is the increased expression ofHSP-70 due to noise exposure. This study was conducted to demonstrate curcuminoid as the safe and effective phytopharrnacy in order to prevent the damage of supporting tissues within the cochlear lateral wall which may lead to NIHL viewed from the expression of HSP-70. The samples were 40 Wistar strain white rats (Rattus norvegicus) divided into 8 groups: The control group/Kl , Group 2/K2 noise (+) for 2 weeks, Group 3a/K3a noise (+) 50 mg/day curcul11inoid (+) for 2 weeks, Group 3b/K3b noise (+) 100 mg/day curcunrinoid (+) for 2 weeks, Group 4a/K4a noise (+) 50 mg/day curcuminoid (+) for 2 weeks, untreated for the next 2 weeks, Group 4b/K4b noise (+) 100 mg/day curcunrinoid (+) for 2 weeks, untreated for the next 2 weeks, Group 5a/K5a 50 mg/day curcunrinoid (+) for 2 weeks, noise (+) and 50 mg/day curcull1inoid (+) for tbe next 2 weeks and Group 5b/K5b 100 mg/day curcuminoid (+) for 2 weeks, noise (+) and 100 mg/day curcul11inoid (+) for the next 2 weeks. This study used curcul11inoid derived from Curcuma /onga L. (Turmeric) with cllrcuminoid content levels [28.1 ± 1.0]% w/w compared to Standard. All samples were examined for the expressions of HSP-70 in cochlear fibroblasts by lmmunohistochemistry method. The data were processed with Analysis of Variance (ANOVA) using the Statistical Analysis System (SAS). The results obtained sbowed significant differences for the expressions of HSP-70 (p<0.05) in all treatment groups, except in Group 3a and 3b. Curcuminoid proved to be potentially effective in the prevention and treatment for the damage of supporting tissues within the cochlear lateral wall regarding tbe decreased expression of HSP-70.