Cochlear Fibroblasts Repairment In Rat Model Diabetes Mellitus As A Results Of Curcumin Treatment (Preliminary Study)
Haryuna, Tengku Siti Hajar
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Background Previous research suggests that curcumin is able to decrease malondialdehyde (MDA) expression due to oxidative stress related to noise exposure on fibroblasts of cochlear lateral wall. The literature shows that the damage of fibroblasts on cochlear lateral wall in diabetes mellitus is happened in similar manner to the damage caused by noise exposure. Therefore, this study aims to prove that curcumin also able to reduce MDA expression on fibroblast of cochlear lateral wall of diabetes mellitus rat models. Materials and Methods This preliminary study conducted on 9 male Wistar strain Rattus norvegicus rats which were divided into 3 groups. Group I serve as the control group which received intraperitoneal injection of citrate buffer and oral CMC through nasogastric tube. Group 2 is diabetes mellitus group model which received streptozotocin injection at a dose of 60mg / kg b.w single dose, administered intraperitoneally. Group 3 received the same treatment as group 2, followed by curcumin treatment with a dose 400 mg / kg b.w / day for 8 days. All rats underwent termination and necropsy procedure on their temporal bones for immunohistochemical assay to detennine the expressions of MDA. Result The increased expression ofMDA was detected in the diabetic group (without curcumin treatment). Curcumin treatment decreased the expression of MDA significantly (p <0.005). Conclusion Curcumin could repair the cochlear damage related to oxidative stress due to diabetes mellitus via decreased expressions of MDA on cochlear fibroblasts in rat models of diabetes mellitus.