Combination effect of ethylacetate extract of Plectranthus amboinicus (Lour.) Spreng. With doxorubicin againts HeLa cell lines
Hasibuan, Poppy Anjelisa Z.
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The aims of the study were to investigate the growth-inhibiting mediating effect of Plectranthus amboinicus (Lour.) Spreng. ethylacetate extract (PAE) in combination therapy with doxorubicin againts HeLa cell lines, to analyzed the apoptotic induction and expression of cyclin D1, Bcl2 and COX-2 (cyclooxigenase-2) proteins of HeLa cell lines after treatment of PAE. The cytotoxicity effects were determined by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis on HeLa cell lines were observed by flowcytometry assay in single dose of PAE. The expression of cyclin D1, Bcl2 and COX-2 proteins of HeLa cell lines after treatment of PAE were identified by using immunocytochemistry. The result showed that PAE had strong synergistic effect with doxorubicin againts HeLa cells based on Combination Index analysis. PAE 28.66 μg/mL increases apoptotic induction. The PAE showed suppression of cyclin D1, Bcl2 and COX-2 expressions on HeLa cell lines. The results concluded that PAE could be a potential co-chemoterapeutic agent with doxorubicin on cervix cancer cells. © 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved.