The activity of Rhaphidophora pinnta Lf. Schott leaf on MCF-7 cell line
Nasution, Maratua Pandapotan
MetadataShow full item record
Ekor naga’s leaf (Rhaphidophora pinnata (Lf) Schott) is a type of vines and climbing plant. The leaves are elongated round and hollowed inside. This plant had been using for the treatment of breast cancer. Extrac- tion with percolation method has been done in ekor naga’s leaves with ethanol, and fractionated by n- hexane, chloroform and ethyl acetate using liquid- liquid extraction (LLE). Cytotoxicity assay of ethanol extract, n-hexane fraction, chloroform fraction, ethyl acetate fraction and water fraction against MCF-7 cells were done using MTT method (3-(4,5-dimetilti- azol-2-il)-2,5-diphenyl tetrazolium bromide). Phyto- chemical screening results showed the presence of the compounds such as triterpenoida/steroid, alkaloid, flavonoid, tannin, and saponin. n-hexane fraction was positive for the presence of triterpenoida/steroid, chlo- roform fraction containing alkaloids, saponin and tri- terpenoid; ethyl acetate fraction contained, flavonoid, tannin, and the fraction of water indicated the pres- ence of tannin and saponin. Secondary metabolite com- pounds in ethanol extract, chloroform fraction and ethyl acetate fraction gave positive results against MCF-7 cells. Cytotoxicity assay of MCF-7 cell line showed that crude ethanol extracts had 112.240 mcg/ml IC50 chloroform fraction IC50 was 59.082 mcg/ml, and ethyl acetate fraction IC50 was 812.663 mcg/ml.