Cardioprotective Effect Of Ethylacetate Extract Of Zanthoxylum acanthopodium DC. Against Doxorubicin-Induced Cardiotoxicity in Rats
Hasibuan, Poppy Anjelisa Z.
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This study was carried out to investigate phytochemical screening of ethylacetate extract of Zanthoxylum acanthopodium DC Lour. fruit (EEZ) and cardioprotective effect against doxorubicin-induced cardiomyopathy in rats. EEZ was prepared by maceration and 300 mg/kg bw as dosage of extract, then cardioprotective effects against doxorubicin (DOX) induced cardiotoxicity was evaluated. DOX was administered to rats at dose of 20 mg/bw through intraperitoneal route for two days. Cardioprotective effect was evaluated by measuring biomarkers troponin T (cTnT), CK-MB levels and histopathology of rat’s heart tissue was examined. Result of phytochemical screening of extract was found to contain alkaloids, flavonoids, tannin, glycosides, and saponin. Levels of cTnT and CK-MB of DOX group differ significantly from the control group, EEZ, EEZ + DOX, vitamin E + DOX, and rutine + DOX (p <0.05). DOX raised cTnT and CK-MB levels significantly (p<0,05) and were counteracted by administration of vitamin E, rutin, and EEZ. Histopathological analysis of rat’s heart tissue resulted in myocytolysis with congestion of blood vessels, pyknosis, cytoplasmic vacuolization and fragmentation. Concomitant treatment with vitamin E, rutin, and EEZ revealed normal muscle fiber. This results suggest that EEZ has cardioprotective effect.