Cardioprotective Effect of Ethylacetate Extract of Poguntano (Picria fel-terrae Lour.) Against Doxorubicin-Induced Cardiotoxicity in Rats
Hasibuan, Poppy Anjelisa Z.
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This study was carried out to investigate phytochemical screening of ethylacetate extract of Picria fel-terrae Lour. leaves (EEP) and cardioprotective effect against doxorubicin-induced cardiomyopathy in rats. Dry EEP was prepared from dry grounded leaves by cold maceration, and extract was given at dosage of 300 mg/kg bw, then it’s cardioprotective effects against doxorubicin (DOX) induced cardiotoxicity was evaluated. DOX was administered intraperitoneally to rats at dosage of 20 mg/bw once a day for two days. Cardioprotective effect was evaluated by measuring biomarkers troponin T (cTnT), CK-MB levels. Histopathology of rat’s heart tissue was also examined. Result of phytochemical screening of extract was found to contain flavonoids, tannins, glycosides, and saponins. Levels of cTnT and CK-MB in DOX group are significantly different from that in control group, EEP, EEP + DOX, vitamin E + DOX, and rutine + DOX (p <0.05). DOX significantly raised cTnT and CK-MB levels (p<0,05) but decreased after administration of vitamin E, rutin, and EEP. Histopathological analysis of rat’s heart tissue showed to resulted in myocytolysis with congestion of blood vessels, pyknosis, cytoplasmic vacuolization and fragmentation. Concomitant treatment with vitamin E, rutin, and EEP revealed normal muscle fiber. These results suggest that EEP has cardioprotective effect.