Badan-Badan Kecil Berbentuk Oval Gelap Di Dalam Kelompokan Makrofag Dan Bercak-Bercak Gelap : Dua Struktur Terabaikan Dalam Diagnosis Limfadenitis Tuberkulosis

Abstract

Background. Cytological diagnose of tuberculous lymphadenopathy is usually based on finding of epitheloid cells and (or) Langhans cells. In some aspirates of lymph nodes stained with May Grunewald Giemsa being not identified the two kinds of the cells but observed dark shrunken oval bodies in the collections of macrophages, and dark specks, it is could not be treated with ordinary antibiotics, but with antituberculous drugs. It needs to investigate whether or not the collections and the dark specks contain tuberculous antigen. To prove that the two structures are the products of tuberculous, immunocytochemistry is performed particularly directed to the two structures. Methods. This research constitute analytic research with cross sectional design. A hundred cases of cytologically suspected tuberculous lymphadenopathy with dark shrunken oval bodies in the collections of macrophages, and dark specks, which will then compared with non specific lymphadenitis include chronic inflammatory, and abscess, which clearly is not a tuberculous infection, the expression of their antigen will be examined by rabbit polyclonal to Mycobacterium tuberculosis antibody (ab905), Abcam. Result. The expression of Mycobacterium tuberculosis is observed on 14 cases with dark shrunken oval bodies in the collections of macrophages, on 21 cases with dark specks, on 1 cases with non specific chronic inflammatory, and on 7 cases with abscess, by rabbit polyclonal to Mycobacterium tuberculosis antibody (ab905), Abcam. Diagnostic test for lesion with cytological feature as dark shrunken oval bodies in the collections of macrophages compared with gold standard is response of antituberculous therapy get sensitivity is 86%, specificity is 50%, posititive predictive value is 86%, negative predictive value is 50%, and prevalence is 78%. While for lesion with cytological feature as dark specks compared with response antituberculous therapy get sensitivity is 95%, specificity is 75%, positive predictive value is 95%, negative predictive value is 75%, and prevalence is 84%. Conclusions. Dark shrunken oval bodies in the collections of macrophages, and dark specks, are two new structures that can be recognized to diagnose tuberculous lymphadenopathy.