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| Title: | Pertubuhan Eksplan Kotileon Jeruk Keprok ( Citrus Nobilis Lour.) Dengan Kultur In Vitro Pada Media MS (Murahige & Skoog) Dengan BAP (Benzyl Amino Purin) |
| Authors: | Seprianti, Reny |
| Advisors: | Rahayu, Suci Nurwahyuni, Isnaini |
| Issue Date: | 14-Jun-2011 |
| Abstract: | Kultur kotiledon telah dilakukan di Laboratorium Kultur Jaringan, Departemen Biologi FMIPA Universitas Sumatera Utara. Kultur Jaringan jeruk Keprok menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan perlakuan Benzyl amino Purin (BAP) 5 konsentrasi yaitu 0, 1, 2, 3 dan 4 ppm. Perlakuan dilakukan 6 ulangan dengan lama 4 minggu. Pengamatan dilakukan dari minggu pertama sampai minggu keempat. Hasil penelitian menunjukkan bahwa persentase keberhasilan kultur kotiledon adalah 75%. Pengamatan pada akhir percobaan menunjukkan konsentrasi pemberian BAP tidak berpengaruh nyata terhadap panjang tunas ,berat planlet, jumlah daun, dan jumlah tunas (P<0.05). Namun demikian ada kecendrungan pertumbuhan pada variabel tersebut. Pada variabel pengamatan untuk panjang akar terdapat pada perlakuan B0 (tanpa penambahan BAP) yang cendrung meningkat, dan pada variabel pengamatan jumlah akar didapatkan jumlah yang homogen yaitu akar keseluruhan berjumlah satu. |
| Abstract (other language): | Cotyledon culturing has been done in the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, University of North Sumatera. The tissue culturing was done using non-factorial Complete Random Design with the treatment using Benzylaminopurin (BAP) in 5 different concentrations, which were 0, 1, 2, 3, and 4 ppm respectively. Every treatment was repeated for six times and observed for four weeks. The result of the research showed that the success percentage of the cotyledon culturing were 75%. The BAP concentration of 4 ppm was the best concentration for the root length and bud length growth, 2 ppm was the best concentration for the bud quantity growth, 0 ppm (control) was the best for the root quantity growth and 1 ppm was the best for the planlet growth. |
| Keywords: | jeruk keprok (Citrus nobilus Lour.) BAP (Benzyl Amino Purin) kultur jaringan |
| URI: | http://repository.usu.ac.id/handle/123456789/26055 |
| Appears in Collections: | SP - Biology
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