Is Transposition Really Random?
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To characterize bacteria, transposon mutagenesis is still one of the most extensively utilized techniques available. These elements were believed to insert at random location. In this study, transposition was done by diparental mating technique to transfer pJFF350 carrying Omegon-Km to a Gram-negative Serratia marcescens DS-8. The result showed that diparental mating was successfully transfer pJFF350 into DS-8 cells. Interestingly, Southern hybridization analyses showed that transposon was inserted not randomly, but tended to insert into limited targets. It also indicated that duplication occurred on the target sequences upon insertion.