Uji Aktivitas Antibakteri Ekstrak Metanol Bunga Rosella (Hibiscus sabdariffa L) Terhadap Bakteri Escherichia coli dan Stapylococcus aureus

Abstract

The study of antibacterial methanolic extract of Rosella flower (Hibiscus sabdariffa L.) to the growth of Escherichia coli and Stapylococcus aureus, was conducted. Agar diffusion method was used in this study. Treatment used was methanolic extract of Roselle flower concentration which were 10 %, 20%, 30%, 40% and 50% whereas cloramfenicol 30 µg was used as a comparison. The results showed that methanolic extracts of roselle flower have different activity in inhibiting the growth of Escherichia coli and Stapylococcus aureus. The methanolic extract of roselle flower showed the highest activity in inhibiting the growth of Escherichia coli was shown by 10% of extract concentration with clear zone diameter was 2,00 mm while Stapylococcus aureus was inhibited by 20% of extract concentration with clear zone diameter was 2,83 mm. These result were lower than inhibition zone caused by cloramfenicol which was 18 mm.

Telah dilakukan penelitian untuk mengetahui Aktivitas Antibakteri Ekstrak Metanol Bunga Rosella (Hibiscus sabdariffa L) terhadap bakteri Escherichia coli dan Stapylococcus aureus.. Pengujian menggunakan metode difusi cakram. Perlakuan yang digunakan adalah konsentrasi ekstrak metanol bunga Rosella yang meliputi 10 %, 20%, 30%, 40% dan 50% sebagai pembanding digunakan antibiotik kloramfenicol 30µg. Hasil uji pengaruh ekstrak metanol bunga Rosella menunjukkan aktivitas yang berbeda dalam menghambat bakteri Escherichia coli dan Stapylococcus aureus. Ekstrak metanol bunga Rosella lebih aktif dalam menghambat bakteri Escherichia coli yaitu pada konsentrasi 10% dengan diamaeter zona bening 2,00 mm sedangkan terhadap bakteri Stapylococcus aureus mulai dihambat pada konsentrasi 20% dengan diameter zona bening 2,83 mm. Aktivitas antibakteri ekstrak metanol bunga Rosella masih lebih kecil dibandingkan antibiotik kloramfenicol dengan diameter hambatan 18 mm.