Determination Of The Aflatoxin B1 In Ground Nut By Differential Pulse Cathodic Stripping Voltammetry (Dpcsv) Technique
Yaacob, Mohammad Hadzri
Yusoff, Abdull Rahim Hj. Mohd.
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An electro analytical method has been developed for the detection and determination of the 2,3,6a,9a-tetrahydro- 4-methoxycyclo penta[c] furo[3’,2’:4,5] furo [2,3-h][l] benzopyran-1,11-dione (aflatoxin B1, AFB1) by a differential pulse cathodic stripping voltammetry on a hanging mercury drop electrode (HMDE) in aqueous solution with Britton-Robinson buffer (BRb) as supporting electrolyte. Effect of instrumental parameters such as accumulation potential (Eacc), accumulation time (tacc) and scan rate (v) were examined. The best condition were found to be pH 9.0, Eacc of -0.6 V, tacc of 80 s and v; 50 mV/s. Calibration curve is linear in the range of 0.02 to 0.32 uM with a detection limit of 0.75 x 10-8 M (3SDblank/m). Relative standard deviation for a replicate measurements of AFB1 ( n= 8) with the concentrations of 0.02 and 0.20 uM were 1.27% and 0.83% respectively. The method is applied for determination of the AFB1 in ground nut samples after extraction and clean-up steps. The recovery values obtained in spiked ground nut sample are 90.93 +/- 2.53 % for 3.0 ppb and 86.15 +/- 2.03 % for 15.0 ppb of AFB1.