Extraction of Bacterial Quinones from Various Different Samples by Using Supercritical CO2
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Supercritical carbon dioxide (scCO2) extraction was applied to various different activated sludge and soil samples in order to clarify the applicability of this technique to environmental samples. The quinone composition of four sludge samples (consisting plant derived activated sludge and domestic waste) and two soil samples were extracted by scCO2 extraction and then analyzed by HPLC. The selected bacterial quinones extracted from the samples by using scCO2 extraction and conventional methods (liquid solvent extraction) were also compared. ScCO2 extraction performed equally as well as the conventional method in extracting a number of bacterial quinones from activated sludge. This experiment confirmed the applicability of scCO2 extraction to extract bacterial quinones from activated sludge obtained from industrial waste water treatment and from soil and compost as well.